Distinct dendritic cell subsets exist as rare populations in lymphoid organs, and therefore are challenging to isolate in sufficient numbers and purity for immunological experiments. Here we describe a high efficiency, high yield method for isolation of all of the currently known major subsets of mouse splenic dendritic cells.
Arora, P., Porcelli, S. A. An Efficient and High Yield Method for Isolation of Mouse Dendritic Cell Subsets. J. Vis. Exp. (110), e53824, doi:10.3791/53824 (2016).