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Einrichtung von Genom bearbeitet humanen pluripotenten Stammzelllinien: Von Targeting zur Isolation
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation
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Einrichtung von Genom bearbeitet humanen pluripotenten Stammzelllinien: Von Targeting zur Isolation

13,248 Views

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09:51 min

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February 02, 2016

DOI:

10.3791/53583-v

DOI:
10.3791/53583-v

•

09:51 min
February 02, 2016

•

13 Views
, ,
1Department of Molecular and Cell Biology,University of California, Berkeley

Summary

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Genome editing of human pluripotent stem cells (hPSCs) can be done quickly and efficiently. Presented here is a robust experimental procedure to genetically engineer hPSCs as exemplified by editing the AAVS1 safe harbor locus to express EGFP and introduce antibiotic resistance.

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Blair, J. D., Bateup, H. S., Hockemeyer, D. F. Establishment of Genome-edited Human Pluripotent Stem Cell Lines: From Targeting to Isolation. J. Vis. Exp. (108), e53583, doi:10.3791/53583 (2016).

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