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L'applicazione di un sistema di espressione inducibile per studiare interferenza di fattori batterici di virulenza con segnalazione intracellulare
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Kapitel
- 00:05Titel
- 01:56Transfection of Stable Cell Line with Inducible Expression Vector System
- 03:08Analysis of Host Cell Apoptosis by Immunoblot Analysis
- 06:41Results: Effects of the Expression of GFP-CaeB on Staurosporine-induced Apoptosis
- 07:46Conclusion
The method described here is used to induce the apoptotic signaling cascade at defined steps in order to dissect the activity of an anti-apoptotic bacterial effector protein. This method can also be used for inducible expression of pro-apoptotic or toxic proteins, or for dissecting interference with other signaling pathways.
