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Perforerade Patch-clamp inspelning av Mouse Olfactory sensoriska neuroner i Intakt neuroepithelium: Funktionell analys av nervceller som uttrycker en identifierad luktreceptor
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Kapitel
- 00:05Titel
- 01:14Pipette and Solution Preparations
- 03:45Preparing the Olfactory Epithelium
- 06:58Making Recordings
- 08:47Results: Stimulating the MOR23 Receptor of Olfactory Receptor Neurons (ORNs) with Lyral
- 09:43Conclusion
Analyzing the physiological properties of olfactory sensory neurons still faces technical limitations. Here we record them through perforated patch-clamp in an intact preparation of the olfactory epithelium in gene-targeted mice. This technique allows the characterization of membrane properties and responses to specific ligands of neurons expressing defined olfactory receptors.
