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鼠标嗅觉感官神经元的完整神经上皮穿孔膜片钳记录：神经元表达鉴定的气味受体功能分析
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JoVE Journal Neurowissenschaften
Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor
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鼠标嗅觉感官神经元的完整神经上皮穿孔膜片钳记录：神经元表达鉴定的气味受体功能分析

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10:16 min

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July 13, 2015

DOI:

10.3791/52652-v

DOI:
10.3791/52652-v

•

10:16 min
July 13, 2015

•

3 Views
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1UMR Centre des Sciences du Goŭt et de l’Alimentation, CNRS, INRA,Université de Bourgogne

Summary

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Analyzing the physiological properties of olfactory sensory neurons still faces technical limitations. Here we record them through perforated patch-clamp in an intact preparation of the olfactory epithelium in gene-targeted mice. This technique allows the characterization of membrane properties and responses to specific ligands of neurons expressing defined olfactory receptors.

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Olfactory Sensory NeuronsPatch-clamp RecordingOdorant ReceptorGenetically Tagged MiceGFPOlfactory EpitheliumMembrane PropertiesLigand-receptor InteractionsTransduction PathwaysPharmacologyCoding Properties

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Cite this Article

Jarriault, D., Grosmaitre, X. Perforated Patch-clamp Recording of Mouse Olfactory Sensory Neurons in Intact Neuroepithelium: Functional Analysis of Neurons Expressing an Identified Odorant Receptor. J. Vis. Exp. (101), e52652, doi:10.3791/52652 (2015).

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