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使用小鸡
GFP
神经管移植和碳水化合物染料 DiI 注射,在鸡胚胎内对神经囊细胞和血管进行双重标记
JoVE Journal
Entwicklungsbiologie
This content is Free Access.
JoVE Journal
Entwicklungsbiologie
Dual Labeling of Neural Crest Cells and Blood Vessels Within Chicken Embryos Using Chick
GFP
Neural Tube Grafting and Carbocyanine Dye DiI Injection
Please note that all translations are automatically generated.
Click here for the English version.
使用小鸡
GFP
神经管移植和碳水化合物染料 DiI 注射,在鸡胚胎内对神经囊细胞和血管进行双重标记
DOI:
10.3791/52514-v
•
09:57 min
•
May 28, 2015
•
Jean-Marie Delalande
2
,
Nikhil Thapar
,
Alan J. Burns
3
1
Birth Defects Research Centre
,
UCL Institute of Child Health
,
2
Blizard Institute, Centre for Digestive Diseases
,
Queen Mary University of London, Barts and The London School of Medicine and Dentistry
,
3
Department of Clinical Genetics
,
Erasmus University Medical Center, Rotterdam
Kapitel
00:05
Titel
01:28
Preparing the Host Embryo
03:30
Preparing the Donor Graft Tissue
05:05
Grafting the Tissue
06:11
Injecting Dil into Blood Vessels
07:20
Harvest Embryos for Sectioning or Wholemount Examination
08:05
Results: Dual Labeling in Chimeric Chick Embryos
09:19
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
在这里,我们报告神经波峰细胞和血管的双重标签使用小鸡
GFP
神经管内部嫁接结合血管内DII注射。这种实验技术使我们能够同时在器官发生期间可视化和研究NCC衍生(肠)神经系统和血管系统的发展。
Tags
Neural Crest Cells
Blood Vessels
Chicken Embryos
ChickGFP Neural Tube Grafting
Carbocyanine Dye DiI Injection
Nervous System
Vascular System
Enteric Nervous System
Chick Chimeras
Transgenic ChickGFP
Quail-chick Interspecies Grafting
Cell Development
Article
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