Mature adipocytes may represent an abundant source of stem cells through dedifferentiation, which leads to a homogenous population of fibroblast-like cells. Collagenase digestion is used to isolate mature adipocytes from human fat. The goal of our protocol is to obtain multipotent, dedifferentiated fat cells from human mature adipocytes.
Lessard, J., Côté, J. A., Lapointe, M., Pelletier, M., Nadeau, M., Marceau, S., Biertho, L., Tchernof, A. Generation of Human Adipose Stem Cells through Dedifferentiation of Mature Adipocytes in Ceiling Cultures. J. Vis. Exp. (97), e52485, doi:10.3791/52485 (2015).