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Tesisinden Akışkan apoplastik çıkarımı için infiltrasyonu-santrifüj Tekniği Kullanarak bırakır<em> Phaseolus vulgaris</em> Bir örnek olarak
JoVE Journal
Biologie
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JoVE Journal Biologie
The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
DOI:

10:26 min

December 19, 2014

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Kapitel

  • 00:05Titel
  • 01:28Leaf Infiltration Using a 60 ml Syringe
  • 03:51Leaf Infiltration by Vacuum Flask
  • 05:13Recovery of Apoplastic Washing Fluid by Centrifugation
  • 06:45Calculation of the Apoplast Dilution Factor
  • 09:02Results: An Example of Metabolite Analysis Made Possible by Apoplast Washing Fluid Extraction
  • 09:45Conclusion

Summary

Automatische Übersetzung

This protocol details the optimized extraction of apoplast washing fluid from plant leaves, using French bean plants (Phaseolus vulgaris) as a model example.

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