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渗透离心技术质外体中提取植物液使用叶<em>菜豆</em>为例
JoVE Journal
Biologie
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JoVE Journal Biologie
The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example
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渗透离心技术质外体中提取植物液使用叶<em>菜豆</em>为例

35,996 Views

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10:26 min

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December 19, 2014

DOI:

10.3791/52113-v

DOI:
10.3791/52113-v

•

10:26 min
December 19, 2014

•

44 Views
, , , ,
1Department of Plant Sciences,University of Oxford, 2School of Education of Vitoria-Gasteiz,University of the Basque Country (UPV/EHU), 3Biosciences,University of Exeter

Summary

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This protocol details the optimized extraction of apoplast washing fluid from plant leaves, using French bean plants (Phaseolus vulgaris) as a model example.

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ApoplastApoplastic FluidApoplast Washing FluidInfiltration-centrifugationPlant LeavesPhaseolus VulgarisCell WallExtracellular CompartmentPlant-endophyte InteractionsPlant Defense Responses

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Cite this Article

O'Leary, B. M., Rico, A., McCraw, S., Fones, H. N., Preston, G. M. The Infiltration-centrifugation Technique for Extraction of Apoplastic Fluid from Plant Leaves Using Phaseolus vulgaris as an Example. J. Vis. Exp. (94), e52113, doi:10.3791/52113 (2014).

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