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Isolatie van menselijke mesenchymale stamcellen en hun kweek op de poreuze botmatrix
JoVE Journal
Entwicklungsbiologie
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JoVE Journal Entwicklungsbiologie
Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix
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Isolatie van menselijke mesenchymale stamcellen en hun kweek op de poreuze botmatrix

27,111 Views

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09:00 min

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February 09, 2015

DOI:

10.3791/51999-v

DOI:
10.3791/51999-v

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09:00 min
February 09, 2015

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15 Views
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1Depto. Materiales Metálicos y Cerámicos, Instituto de Investigaciones en Materiales,Universidad Nacional Autónoma de México (UNAM), 2Depto. of Microbiología y Parasitología, Facultad de Medicina,Universidad Nacional Autónoma de México (UNAM), 3Depto. Biología Celular y Tisular, Facultad de Medicina,Universidad Nacional Autónoma de México (UNAM), 4Depto de Biología Celular, Facultad de Química,Universidad Nacional Autónoma de México (UNAM), 5Depto. Genetica y Biología Molecular,Cinvestav-IPN, 6Subd. de Investigación,Centro Nacional de la Transfusión Sanguínea, Secretaria de Salud

Summary

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Mesenchymal stem cells (MSCs) have a differentiation potential towards osteoblastic lineage when they are stimulated with soluble factors or specific biomaterials. This work presents a novel option for the delivery of MSCs from human amniotic membrane (AM-hMSCs) that employs the bovine bone matrix Nukbone (NKB) as a scaffold.

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Mesenchymal Stem CellsAM-hMSCsBone MatrixNukboneScaffoldCell DifferentiationBiomaterialsCell Culture

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Rodríguez-Fuentes, N., Reynoso-Ducoing, O., Rodríguez-Hernández, A., Ambrosio-Hernández, J. R., Piña-Barba, M. C., Zepeda-Rodríguez, A., Cerbón-Cervantes, M. A., Tapia-Ramírez, J., Alcantara-Quintana, L. E. Isolation of Human Mesenchymal Stem Cells and their Cultivation on the Porous Bone Matrix. J. Vis. Exp. (96), e51999, doi:10.3791/51999 (2015).

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