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एक प्रतिबंध एंजाइम आधारित क्लोनिंग विधि का आकलन करने के लिए<em> इन विट्रो</em> एचआईवी -1 उपप्रकार सी चुप-MJ4 काइमेरिक वायरस की प्रतिकृति क्षमता
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Kapitel
- 00:05Titel
- 01:53Amplification of the HIV-1 Gag Gene from Infected, Frozen Plasma
- 03:29Cloning Amplified Gag Genes into the MJ4, Subtype C, Infectious Molecular Clone
- 06:09In vitro Replication of Gag-MJ4 Chimeras in GXR25 (CEM-CCR5-GFP) Cells
- 10:06Analysis of Reverse Transcriptase in Cell Culture Supernatants
- 12:11Results: A Restriction Enzyme Based Cloning Method to Assess the In vitro Replication Capacity of HIV-1 Subtype C Gag-MJ4 Chimeric Viruses
- 13:44Conclusion
HIV-1 pathogenesis is defined by both viral characteristics and host genetic factors. Here we describe a robust method that allows for reproducible measurements to assess the impact of the gag gene sequence variation on the in vitro replication capacity of the virus.
