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Immunology and Infection

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JoVE Journal Immunology and Infection
Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish
 

Quantification of the Respiratory Burst Response as an Indicator of Innate Immune Health in Zebrafish

Article DOI: 10.3791/50667-v • 07:07 min • September 12th, 2013
September 12th, 2013 •
1, 1, 1, 1
1Department of Molecular and Biomedical Sciences, University of Maine

Kapitel

Summary
Automatic Translation

The innate immune response protects organisms against pathogen infection. A critical component of the innate immune response, the phagocyte respiratory burst, generates reactive oxygen species that kill invading microorganisms. We describe a respiratory burst assay that quantifies reactive oxygen species produced when the innate immune response is chemically induced.

Tags

Respiratory Burst Response Innate Immune Health Zebrafish Phagocyte Reactive Oxygen Species (ROS) Quantification Chemical Induction Fluorogenic Substrate Microplate Reader Fluorescence Readings Developmental Stages Experimental Manipulations Protein Knockdown Overexpression Pharmacological Agents
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