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Forward Genetic Approaches in Chlamydia trachomatis
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Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Forward Genetic Approaches in Chlamydia trachomatis

Forward Genetic Approaches in Chlamydia trachomatis

12,658 Views

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09:03 min

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October 23, 2013

DOI:

10.3791/50636-v

DOI:
10.3791/50636-v

•

09:03 min
October 23, 2013

•

3 Views
,
1Department of Molecular Genetics and Microbiology, Center for Microbial Pathogenesis,Duke University Medical Center

Summary

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We describe a methodology to perform genetic analysis in Chlamydia based on chemical mutagenesis and whole genome sequencing. In addition, a system for DNA exchange within infected cells is described that can be used for genetic mapping. This method may be broadly applicable to microbial systems lacking transformation systems and molecular genetic tools.

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Genetic AnalysisChlamydia TrachomatisForward GeneticsChemical MutagenesisWhole-genome SequencingGenotype-phenotype AssociationRecombinant StrainsGenetic ToolsIntractabilitySexually Transmitted DiseasesOcular Infections

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Cite this Article

Nguyen, B. D., Valdivia, R. H. Forward Genetic Approaches in Chlamydia trachomatis. J. Vis. Exp. (80), e50636, doi:10.3791/50636 (2013).

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