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Forward Genetic Approaches in Chlamydia trachomatis
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Forward Genetic Approaches in Chlamydia trachomatis

Forward Genetic Approaches in Chlamydia trachomatis

DOI:
10.3791/50636-v

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09:03 min

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October 23, 2013

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,
1Department of Molecular Genetics and Microbiology, Center for Microbial Pathogenesis,Duke University Medical Center

Chapters

  • 00:05Title
  • 01:20Chemical Mutagenesis
  • 03:49Clonal Isolation of Chlamydia trachomatis Mutant Strains by Plaque Purification
  • 07:14Representative Chlamydia Mutagenesis Results
  • 08:29Conclusion

Summary

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We describe a methodology to perform genetic analysis in Chlamydia based on chemical mutagenesis and whole genome sequencing. In addition, a system for DNA exchange within infected cells is described that can be used for genetic mapping. This method may be broadly applicable to microbial systems lacking transformation systems and molecular genetic tools.

Tags

Genetic AnalysisChlamydia TrachomatisForward GeneticsChemical MutagenesisWhole-genome SequencingGenotype-phenotype AssociationRecombinant StrainsGenetic ToolsIntractabilitySexually Transmitted DiseasesOcular Infections

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