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A Caenorhabditis elegans Model System for Amylopathy Study
Kapitel
- 00:05Titel
- 01:25Transformation of Transgenic Worms
- 04:24Age-synchronization
- 05:39Chemotaxis Assay
- 06:44Primary Embryonic Cell Culture
- 08:51The Effects of Human Aβ42 Oligomer on Neuronal Function
- 10:11Conclusion
We describe methods to study aspects of amylopathies in the worm C. elegans. We show how to construct worms expressing human Aβ42 in neurons and how to test their function in behavioral assays. We further show how to obtain primary neuronal cultures that can be used for pharmacological testing.
