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En molekylær Avlesning av Long-term olfactory Tilpasning i<em> C. elegans</em
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JoVE Journal Neurowissenschaften
A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

En molekylær Avlesning av Long-term olfactory Tilpasning i<em> C. elegans</em

DOI:
10.3791/4443-v

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11:30 min

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December 22, 2012

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, , ,
1Department of Biological Sciences and Institute for Neuroscience,George Washington University, 2Fred Hutchinson Cancer Research Center, 3Department of Cell and Tissue Biology,University of California San Francisco

Kapitel

  • 00:05Titel
  • 01:57Cultivation and Maintenance of Animals for Nuclear Translocation Assays
  • 03:26Long-term Odor Induced Nuclear Translocation Assays
  • 05:44Scoring the Odor-induced Nuclear Translocation Event
  • 08:54Results: Molecular Readout of Long-term Olfactory Adaptation in C. elegans
  • 11:09Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Her beskriver vi en molekylær avlesning av langsiktig olfactory tilpasning i<em> Caenorhabditis elegans</em>. Proteinkinase G, EGL-4, som er nødvendig for stabile tilpasningstiltak responser i primær sensorisk neuron pair kalt AWC. Under langvarig lukt eksponering EGL-4 translocates fra cytosol til kjernen av AWC.

Tags

Molecular ReadoutLong-term Olfactory AdaptationC. ElegansSensory NeuronsAdaptation ResponseOlfactory CircuitGenetic ScreenMutantsVolatile OdorsAmphid Wing Cells Type C (AWC) Sensory NeuronsChemotaxis Behavioral AssayShort-term Olfactory AdaptationLong-term Olfactory AdaptationProtein Kinase G (PKG)EGL-4 Protein

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