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A Molecular Readout of Long-term Olfactory Adaptation in C. elegans
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JoVE Journal Neurowissenschaften
A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

A Molecular Readout of Long-term Olfactory Adaptation in C. elegans

DOI:
10.3791/4443-v

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11:30 min

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December 22, 2012

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, , ,
1Department of Biological Sciences and Institute for Neuroscience,George Washington University, 2Fred Hutchinson Cancer Research Center, 3Department of Cell and Tissue Biology,University of California San Francisco

Kapitel

  • 00:05Titel
  • 01:57Cultivation and Maintenance of Animals for Nuclear Translocation Assays
  • 03:26Long-term Odor Induced Nuclear Translocation Assays
  • 05:44Scoring the Odor-induced Nuclear Translocation Event
  • 08:54Results: Molecular Readout of Long-term Olfactory Adaptation in C. elegans
  • 11:09Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Here we describe a molecular readout of long-term olfactory adaptation in Caenorhabditis elegans. The Protein Kinase G, EGL-4, is necessary for stable adaptation responses in the primary sensory neuron pair called AWC. During prolonged odor exposure EGL-4 translocates from the cytosol to nucleus of the AWC.

Tags

Molecular ReadoutLong-term Olfactory AdaptationC. ElegansSensory NeuronsAdaptation ResponseOlfactory CircuitGenetic ScreenMutantsVolatile OdorsAmphid Wing Cells Type C (AWC) Sensory NeuronsChemotaxis Behavioral AssayShort-term Olfactory AdaptationLong-term Olfactory AdaptationProtein Kinase G (PKG)EGL-4 Protein

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