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A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting

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07:27 min

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May 16, 2013

DOI:

10.3791/4316-v

DOI:
10.3791/4316-v

•

07:27 min
May 16, 2013

•

3 Views
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1Malawi-Liverpool-Wellcome Trust Clinical Research Programme, 2Liverpool School of Tropical Medicine, 3Department of Microbiology, Division of Medical Parasitology,New York University School of Medicine

Summary

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This method investigates the platelet-mediated clumping phenotype of Plasmodium falciparum-infected erythrocytes (pRBC) in clinical isolates. This is performed by isolating and co-incubating platelet-rich plasma and a suspension of pRBC.

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Tembo, D. L., Montgomery, J., Craig, A. G., Wassmer, S. C. A Simple Protocol for Platelet-mediated Clumping of Plasmodium falciparum-infected Erythrocytes in a Resource Poor Setting. J. Vis. Exp. (75), e4316, doi:10.3791/4316 (2013).

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