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Kapitel
- 00:05Titel
- 01:34Experiment Day 0: Sample Preparation and ELISA Test Plate Coating with Capture Antibody for IL-2Ralpha, REG3alpha and HGF
- 02:48Experiment Day 1: IL-2Ralpha and REG3alpha ELISAs
- 04:13Experiment Day 1: Elafin and TNFR1 Test Plate Coating with Capture Antibody
- 04:39Experiment Day 1-2: HGF ELISA
- 05:46Experiment Day 2: Elafin ELISA
- 06:16Experiment Day 2: TNFR1 ELISA
- 06:45Experiment Day 2: IL-8 ELISA
- 07:25Results: Representative Optical Densities for the Standard Curve of 7 Different ELISA Plates Measuring REG3alpha Concentrations
- 08:06Conclusion
Convalida elevato throughput di biomarcatori candidati multipli può essere eseguita da ELISA sequenziale per minimizzare gelo / disgelo e l'uso di campioni di plasma preziosi. Qui, dimostriamo come eseguire in sequenza ELISA per sei diversi biomarcatori plasmatici convalidati<sup> 1-3</sup> Di graft-versus-host disease (GVHD)<sup> 4</sup> Il campione di plasma stesso.
Tags
High Throughput Sequential ELISAValidation Of BiomarkersAcute Graft-Versus-Host DiseaseUnbiased Discovery Proteomics StrategiesNovel BiomarkersDiagnostic TestingPrognostic TestingTherapeutic InterventionsCandidate ProteinsValidate Candidate BiomarkersPatient Plasma SamplesAcute Graft-versus-host DiseaseAllogeneic Hematopoietic Stem Cell TransplantationELISAsGVHD ProteinsIL-2Rα5TNFR16HGF7IL-88Elafin2REG3α3PAP1Sequential ELISA PerformanceFreeze-thaw CyclesThawed Plasma TimePlasma UsagePlasma Biomarker Concentrations
