/
/
piggyBac modificação do sistema Transposon de células T primárias Humanos
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
piggyBac modificação do sistema Transposon de células T primárias Humanos
16,936 Views
•
10:02 min
•
November 05, 2012
DOI:
1Program in Translational Biology and Molecular Medicine,Baylor College of Medicine , 2Department of Medicine, Division of Nephrology,Baylor College of Medicine , 3Department of Immunology and Pathology,Shinshu University School of Medicine, 4Center for Cell and Gene Therapy,Baylor College of Medicine , 5Department of Pediatrics,Baylor College of Medicine , 6Program in Cell and Molecular Biology,Baylor College of Medicine , 7Department of Molecular Virology and Microbiology,Baylor College of Medicine , 8Michael E. DeBakey VA Medical Center
Summary
Automatically generated
Descreve-se um método para modificar geneticamente células T primárias humanas com um transgene, utilizando o não-viral<em> PiggyBac</em> Sistema de transposon. As células T modificado para utilizar o<em> PiggyBac</em> Transposon sistema mostra a expressão do transgene estável.
Read Article
Cite this Article
Saha, S., Nakazawa, Y., Huye, L. E., Doherty, J. E., Galvan, D. L., Rooney, C. M., Wilson, M. H. piggyBac Transposon System Modification of Primary Human T Cells. J. Vis. Exp. (69), e4235, doi:10.3791/4235 (2012).
Copy