Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich. Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
기본 인간 T 세포 piggyBac Transposon 시스템 변경
16,936 Views
•
10:02 min
•
November 05, 2012
DOI:
1Program in Translational Biology and Molecular Medicine,Baylor College of Medicine , 2Department of Medicine, Division of Nephrology,Baylor College of Medicine , 3Department of Immunology and Pathology,Shinshu University School of Medicine, 4Center for Cell and Gene Therapy,Baylor College of Medicine , 5Department of Pediatrics,Baylor College of Medicine , 6Program in Cell and Molecular Biology,Baylor College of Medicine , 7Department of Molecular Virology and Microbiology,Baylor College of Medicine , 8Michael E. DeBakey VA Medical Center
Summary
Automatically generated
우리는 유전자 비 바이러스를 사용하여 transgene과 기본 인간의 T 세포를 수정하는 방법을 설명<em> piggyBac</em> transposon 시스템입니다. T 세포는를 사용하여 수정<em> piggyBac</em> transposon 시스템 전시 안정적인 transgene 표현.
Read Article
Cite this Article
Saha, S., Nakazawa, Y., Huye, L. E., Doherty, J. E., Galvan, D. L., Rooney, C. M., Wilson, M. H. piggyBac Transposon System Modification of Primary Human T Cells. J. Vis. Exp. (69), e4235, doi:10.3791/4235 (2012).
Copy