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Isolamento e la coltura delle cellule della cresta neurale da Embryonic Murine tubo neurale
JoVE Journal
Neurowissenschaften
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JoVE Journal Neurowissenschaften
Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

Isolamento e la coltura delle cellule della cresta neurale da Embryonic Murine tubo neurale

DOI:

12:48 min

June 02, 2012

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Kapitel

  • 00:05Titel
  • 01:14Introduction
  • 01:36Prepare Plates
  • 02:18Preparation of Self Renewal and Wash Media
  • 03:06Preparation of Collagenase/Dispase
  • 04:22Isolating Vagal and Trunk Neural Tube
  • 08:21Removal of Non-Neural Ectoderm and Mesoderm
  • 11:06Removal of the Neural Tube
  • 11:46Representative Results
  • 12:35Conclusions

Summary

Automatische Übersetzung

Isolamento di embrionale cresta neurale dal tubo neurale facilita l'uso di<em> In vitro</em> Metodi per lo studio della migrazione, self-renewal, e multipotenza di cresta neurale.

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