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从小鼠胚胎神经管的神经嵴细胞的分离和培养

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JoVE Journal Neurowissenschaften

Isolation and Culture of Neural Crest Cells from Embryonic Murine Neural Tube

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从小鼠胚胎神经管的神经嵴细胞的分离和培养

DOI:

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12:48 min

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June 02, 2012

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Elise R. Pfaltzgraff, Nathan A. Mundell², Patricia A. Labosky

¹Department of Cell and Developmental Biology, Center for Stem Cell Biology,Vanderbilt University Medical Center, ²Department of Pharmacology, Center for Stem Cell Biology,Vanderbilt University Medical Center, ³Vanderbilt University Medical Center

Kapitel

00:05Titel
01:14Introduction
01:36Prepare Plates
02:18Preparation of Self Renewal and Wash Media
03:06Preparation of Collagenase/Dispase
04:22Isolating Vagal and Trunk Neural Tube
08:21Removal of Non-Neural Ectoderm and Mesoderm
11:06Removal of the Neural Tube
11:46Representative Results
12:35Conclusions

Summary

Automatische Übersetzung

胚胎神经嵴的神经管分离，便于使用<em>在体外</em>学习迁移，自我更新和多能神经嵴的方法。

Tags

Neural Crest Cells Embryonic Murine Neural Tube Isolation Culture Multipotent Progenitor Population Epithelial To Mesenchymal Transition Migration Self-renewal Avian Neural Tube Rat Systems Explanted NC Cells Proliferation Transplantation Cellular Properties Neural Tube Explant Assay Mammalian NC Isolation Methods Post-migratory NC Progenitors

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