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Immunology and Infection

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JoVE Journal Immunology and Infection
Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue
 

Primary Microglia Isolation from Mixed Glial Cell Cultures of Neonatal Rat Brain Tissue

Article DOI: 10.3791/3814-v • 10:20 min • August 15th, 2012
August 15th, 2012 •
1, 1,2,3, 2
1Neuroscience Program, Uniformed Services University, 2Department of Anatomy, Physiology, and Genetics, Uniformed Services University, 3Molecular and Cell Biology, Uniformed Services University

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Summary
Automatic Translation

Isolating primary microglia from the cellular heterogeneity of the brain is essential to investigate their role in both physiological and pathological conditions. This protocol describes a mechanical isolation and mixed cell culture technique that provides high yield and high purity, viable primary microglial cells for in vitro study and downstream applications.

Tags

Primary Microglia Isolation Mixed Glial Cell Cultures Neonatal Rat Brain Tissue Microglia Role In Brain Physiology Phagocytic Activity Microglial Activation Neurodegenerative Disease Traumatic Brain Injury Nervous System Infection Reactive Oxygen And Nitrogen Species Pro-inflammatory Chemokines And Cytokines Cellular Heterogeneity Of The Brain Neuroprotective And Neurotoxic Functions Of Microglia Isolation Protocol For Primary Microglia
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