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Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
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JoVE Journal Immunologie und Infektion
Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

15,193 Views

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07:56 min

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September 03, 2012

DOI:

10.3791/3673-v

DOI:
10.3791/3673-v

•

07:56 min
September 03, 2012

•

1 Views
, , , ,
1School of Veterinary Medicine and Biomedical Sciences,University of Nebraska-Lincoln, 2Department of Chemistry,University of Nebraska-Lincoln

Summary

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The metabolomic profile of Mycobacterium tuberculosis is determined after growth in broth cultures. Conditions can be varied to test the effects of nutritional supplements, oxidants, and anti-tuberculosis agents on the metabolic profile of this microorganism. Procedure for extract preparation is applicable for both 1D 1H and 2D 1H-13C NMR analyses.

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Mycobacterium TuberculosisNuclear Magnetic ResonanceMetabolomic StudiesSample PreparationDrug-resistant StrainsGene FunctionGene Expression AnalysisGlobal PatternsMetabolic CompositionSmall Molecular Weight ChemicalsTarget IdentificationAnti-tubercular ActivityNMR Technology

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Cite this Article

Zinniel, D. K., Fenton, R. J., Halouska, S., Powers, R., Barletta, R. G. Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies. J. Vis. Exp. (67), e3673, doi:10.3791/3673 (2012).

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