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Immunology and Infection

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JoVE Journal Immunology and Infection
Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies
 

Sample Preparation of Mycobacterium tuberculosis Extracts for Nuclear Magnetic Resonance Metabolomic Studies

Article DOI: 10.3791/3673-v • 07:56 min • September 3rd, 2012
September 3rd, 2012 •
1, 1, 2, 2, 1
1School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, 2Department of Chemistry, University of Nebraska-Lincoln

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The metabolomic profile of Mycobacterium tuberculosis is determined after growth in broth cultures. Conditions can be varied to test the effects of nutritional supplements, oxidants, and anti-tuberculosis agents on the metabolic profile of this microorganism. Procedure for extract preparation is applicable for both 1D 1H and 2D 1H-13C NMR analyses.

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Mycobacterium Tuberculosis Nuclear Magnetic Resonance Metabolomic Studies Sample Preparation Drug-resistant Strains Gene Function Gene Expression Analysis Global Patterns Metabolic Composition Small Molecular Weight Chemicals Target Identification Anti-tubercular Activity NMR Technology
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