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High-throughput Metodo di rilevazione per il virus dell'influenza
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High-throughput Metodo di rilevazione per il virus dell'influenza
1Laboratory of Molecular Immunology and Immunotherapy,Blood Research Institute, 2Department of Microbiology,Mount Sinai School of Medicine , 3Laboratory of Molecular Genetics,Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children’s Hospital of Wisconsin,Medical College of Wisconsin , 6Division of Hematology and Oncology, Dept Medicine,Medical College of Wisconsin
Kapitel
- 00:05Titel
- 02:26Intra-nasal Infection of Mice with Influenza and Collection of Bronchio-Alveolar Lavage Fluid
- 03:22Infection of MDCK Cells with Virus and Detection of Viral M2 Protein with the LI-COR Odyssey
- 08:09Quantification of Influenza Viral Titer using Infra-red Staining and Detection
- 09:16Conclusion
Questo metodo descrive l'utilizzo di Infrared sistema colorante di imaging based per il rilevamento di H1N1 in bronchioalveolar lavaggio (BAL) fluido di topi infettati ad una sensibilità elevata. Questa metodologia può essere eseguita in uno 96 - o 384-bene piastra, richiede <10 volume pl di materiale di prova e ha il potenziale per lo screening concomitante di agenti patogeni multipli.
Tags
High-throughput Detection MethodInfluenza VirusPrecise DiagnosisInfecting StrainRapid ScreeningClinical SamplesPandemic InfectionsSerologic TestingPolymerase Chain ReactionDirect Specimen ImmunofluorescenceCell CultureNovel Diagnostic TechniqueLive Influenza VirusesMouse-adapted Human A/PR/8/34 (PR8H1N1) VirusMDCK CellsViral ProteinsAnti-M2 AntibodyHemagglutinin 1Diagnostic AssaysIR-dye-conjugated Secondary AntibodiesAntigen-specific Fluorescence IntensityLI-COR Odyssey-based IR Scanner
