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인플루엔자 바이러스에 대한 높은 처리량 검출 방법
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10:05 min
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February 04, 2012
DOI:
1Laboratory of Molecular Immunology and Immunotherapy,Blood Research Institute, 2Department of Microbiology,Mount Sinai School of Medicine , 3Laboratory of Molecular Genetics,Blood Research Institute, 4City of Milwaukee Health Department Laboratory, 5Division of Hematology-Oncology/BMT, Children’s Hospital of Wisconsin,Medical College of Wisconsin , 6Division of Hematology and Oncology, Dept Medicine,Medical College of Wisconsin
Summary
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이 방법은 높은 감도에 감염된 생쥐들의 bronchioalveolar 세척 (BAL) 유체에서 H1N1의 탐지를위한 적외선 염료 기반 이미징 시스템의 사용을 설명합니다. 이 방법론은 96에서 수행할 수 있습니다 - 또는 판 384 자, 시험 물질 <10 μl 볼륨이 필요하고 여러 병원체의 동시 상영을위한 잠재력을 가지고 있습니다.
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Cite this Article
Kumar, P., Bartoszek, A. E., Moran, T. M., Gorski, J., Bhattacharyya, S., Navidad, J. F., Thakar, M. S., Malarkannan, S. High-throughput Detection Method for Influenza Virus. J. Vis. Exp. (60), e3623, doi:10.3791/3623 (2012).
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