We have developed novel laboratory tools and protocols for intravital imaging acquisition of the thymus. Our technique should help in the identification of “niches” within the thymus where T cell development occurs.
Caetano, S. S., Teixeira, T., Tadokoro, C. E. Intravital Imaging of the Mouse Thymus using 2-Photon Microscopy. J. Vis. Exp. (59), e3504, doi:10.3791/3504 (2012).