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Bioengineering Human Microvascular Networks in Immunodeficient Mice
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July 11, 2011
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Here, we describe a methodology to deliver human cord blood-derived endothelial colony-forming cells (ECFCs) and bone marrow-derived mesenchymal stem cells (MSCs), embedded in a collagen/fibronectin gel, subcutaneously into immunodeficient mice. This cell/gel combination generates a human vascular network that connects with the mouse vasculature.
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BiotechnikHuman Microvascular NetworksImmunodeficient MiceCell-based TherapiesVascular NetworksIn Vivo ModelsEndothelial CellsAutologous Endothelial CellsClinical UseEndothelial Colony-forming CellsBlood-derived ECsFunctional Vascular NetworksPerivascular CellsCord Blood-derived ECFCsMesenchymal Stem Cells (MSCs)Collagen/fibronectin/fibrinogen Gel
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Lin, R., Melero-Martin, J. M. Bioengineering Human Microvascular Networks in Immunodeficient Mice. J. Vis. Exp. (53), e3065, doi:10.3791/3065 (2011).
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