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Bioengineering Human Microvascular Networks in Immunodeficient Mice
Chapters
- 00:05Title
- 01:00Culture of Human Cord Blood-derived Endothelial Colony-forming Cells (ECFCs) and Bone-marrow Derived Mesenchymal Stem Cells (MSCs)
- 02:51Resuspension of Cells in Collagen/Fibronectin/Fibrinogen Solution (Day 0)
- 04:05Injection into Immunodeficient Nude Mouse (Day 0)
- 04:51Harvesting the Implants (Day 7) and Evaluation
- 05:50Cell Morphology
- 06:38Conclusion
Here, we describe a methodology to deliver human cord blood-derived endothelial colony-forming cells (ECFCs) and bone marrow-derived mesenchymal stem cells (MSCs), embedded in a collagen/fibronectin gel, subcutaneously into immunodeficient mice. This cell/gel combination generates a human vascular network that connects with the mouse vasculature.
Tags
生物工程Human Microvascular NetworksImmunodeficient MiceCell-based TherapiesVascular NetworksIn Vivo ModelsEndothelial CellsAutologous Endothelial CellsClinical UseEndothelial Colony-forming CellsBlood-derived ECsFunctional Vascular NetworksPerivascular CellsCord Blood-derived ECFCsMesenchymal Stem Cells (MSCs)Collagen/fibronectin/fibrinogen Gel
