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Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
JoVE Journal
Medizin
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JoVE Journal Medizin
Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy

DOI:
10.3791/2967-v

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09:44 min

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October 20, 2011

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, , , , , ,
1Department of Orthodontics and Maxillofacial Orthopedics,Medical University of Graz, 2Institute of Hygiene, Microbiology and Environmental Medicine,Medical University of Graz, 3Department of Prosthodontics, Restorative Dentistry, Periodontology and Implantology,Medical University of Graz, 4Institute of Plant Sciences,Karl-Franzens-University Graz

Kapitel

  • 00:05Titel
  • 01:51Specimen Collection and Biofilm Fixation
  • 03:38Dehydration of Fixed Samples
  • 04:34In-situ Hybridization and Imaging
  • 07:21Results: Biofilm Images
  • 08:56Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

We present a protocol for structural and compositional analysis of natural oral biofilm from orthodontic appliances with in situ hybridization (FISH) and confocal laser scanning microscopy (CLSM). Oral biofilm samples were collected from palatal expanders, scraping acrylic-resin flakes off their surface and referring them for molecular processing.

Tags

Oral Biofilm AnalysisPalatal ExpandersFluorescence In Situ Hybridization (FISH)Confocal Laser Scanning Microscopy (CLSM)Staining Techniques16S RRNA-targeting ProbesBiofilm ConsortiaEubacteriaFirmicutesBacteroidetesStreptococcus MutansPorphyromonas GingivalisSurface Materials

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