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Analyse orale de biofilm palatine Expandeurs par fluorescence hybridation in situ et la microscopie confocale à balayage laser
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Medizin
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JoVE Journal Medizin
Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy
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Analyse orale de biofilm palatine Expandeurs par fluorescence hybridation in situ et la microscopie confocale à balayage laser

15,636 Views

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09:44 min

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October 20, 2011

DOI:

10.3791/2967-v

DOI:
10.3791/2967-v

•

09:44 min
October 20, 2011

•

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1Department of Orthodontics and Maxillofacial Orthopedics,Medical University of Graz, 2Institute of Hygiene, Microbiology and Environmental Medicine,Medical University of Graz, 3Department of Prosthodontics, Restorative Dentistry, Periodontology and Implantology,Medical University of Graz, 4Institute of Plant Sciences,Karl-Franzens-University Graz

Summary

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Nous présentons un protocole pour l'analyse structurelle et compositionnelle du biofilm naturel d'administration orale à partir d'appareils orthodontiques<em> In situ</emL'hybridation> (FISH) et microscopie confocale à balayage laser (CLSM). Échantillons de biofilms oraux ont été recueillies auprès de expandeurs palatine, le grattage de résine acrylique s'écaille leur surface et leur référence pour le traitement moléculaire.

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Klug, B., Rodler, C., Koller, M., Wimmer, G., Kessler, H. H., Grube, M., Santigli, E. Oral Biofilm Analysis of Palatal Expanders by Fluorescence In-Situ Hybridization and Confocal Laser Scanning Microscopy. J. Vis. Exp. (56), e2967, doi:10.3791/2967 (2011).

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