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Immunologie und Infektion
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Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

DOI:
10.3791/2785-v

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07:34 min

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June 02, 2011

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, , ,
1Molecular and Cell Biology Program,Ohio University, 2Department of Biomedical Sciences,College of Osteopathic Medicine, Ohio University, 3Department of Biomedical Engineering,Russ College of Engineering and Technology, Ohio University

Kapitel

  • 00:05Titel
  • 01:30Dissection of Mouse Femurs and Tibiae and Culture of Bone Marrow Cells
  • 03:59Collection and Labeling of DCs
  • 05:10Results: Qdot Nanocrystal Staining of DCs
  • 07:04Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Dendritic cells uptake antigens and migrate towards immune organs to present processed antigens to T cells. Qdot nanocrystal labeling provides a long-lasting and stable fluorescent signal. This allows tracking of dendritic cells to different organs by fluorescent microscopy.

Tags

GenerationLabelingMurineBone Marrow-derived Dendritic CellsQdot NanocrystalsTracking StudiesAntigen Presenting CellsImmunological OrgansMajor Histocompatibility MoleculesMigratory CapabilitiesFluorescent DyesQdot Fluorescent NanocrystalsStable FluorescenceCell CultureDifferentiationIn Vitro IncubationFluorescent MicroscopyExperimental AnimalsMature Cells

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