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Geração e Rotulagem de murino derivadas da medula óssea Células Dendríticas com Nanocristais Qdot de Estudos de rastreamento
JoVE Journal
Immunologie und Infektion
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JoVE Journal Immunologie und Infektion
Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies

Geração e Rotulagem de murino derivadas da medula óssea Células Dendríticas com Nanocristais Qdot de Estudos de rastreamento

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07:34 min

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June 02, 2011

DOI:

10.3791/2785-v

DOI:
10.3791/2785-v

•

07:34 min
June 02, 2011

•

7 Views
, , ,
1Molecular and Cell Biology Program,Ohio University, 2Department of Biomedical Sciences,College of Osteopathic Medicine, Ohio University, 3Department of Biomedical Engineering,Russ College of Engineering and Technology, Ohio University

Summary

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Dendríticas células antígenos captação e migrar para órgãos imunes a antígenos presentes processados ​​às células T. Qdot nanocristais rotulagem fornece um sinal duradouro e estável fluorescente. Este permite o rastreamento de células dendríticas a diferentes órgãos, por microscopia fluorescente.

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Cite this Article

Muccioli, M., Pate, M., Omosebi, O., Benencia, F. Generation and Labeling of Murine Bone Marrow-derived Dendritic Cells with Qdot Nanocrystals for Tracking Studies. J. Vis. Exp. (52), e2785, doi:10.3791/2785 (2011).

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