Journal
/
Neurowissenschaften
/
An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival
JoVE Journal
Neurowissenschaften
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Neurowissenschaften
An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival

An Optic Nerve Crush Injury Murine Model to Study Retinal Ganglion Cell Survival

DOI:
10.3791/2685-v

•

09:07 min

•

April 25, 2011

•

, , , , , , ,
1National Eye Institute,NIH, 2Ophthalmology Department,The Second Hospital of Harbin Medical University

Kapitel

  • 00:05Titel
  • 02:25Baseline Retrograde Labeling of Retinal Ganglion Cells (RGCs) at Day 1
  • 04:47Optic Nerve Crush Injury at Day 4 after Fluorescence Dye Injection
  • 06:22Retinal Harvest and Analysis of RGC Survival
  • 07:13Analysis of Retinal Ganglion Cell Survival after Optic Nerve Crush Injury
  • 08:01Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

This protocol shows how to retrogradely label retinal ganglion cells, and how to subsequently make an optic nerve crush injury in order to analyze retinal ganglion cell survival and apoptosis. It is an experimental disease model for different types of optic neuropathy, including glaucoma.

Tags

Optic Nerve Crush InjuryRetinal Ganglion Cell SurvivalOptic NeuropathyGlaucomaVision LossIrreversibleExperimental Disease ModelApoptosisNeuronal Death And SurvivalTherapeutic MeasuresTherapeutic ReagentsRetrograde LabelingHarvest The RetinaeRGC Survival

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Optic Nerve Transection: A Model of Adult Neuron Apoptosis in the Central Nervous System

An Isolated Retinal Preparation to Record Light Response from Genetically Labeled Retinal Ganglion Cells

Minimally-invasive Technique for Injection into Rat Optic Nerve

Visual Evoked Potential Recording in a Rat Model of Experimental Optic Nerve Demyelination

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Dorsal Root Ganglion Injection and Dorsal Root Crush Injury as a Model for Sensory Axon Regeneration

Partial Optic Nerve Transection in Rats: A Model Established with a New Operative Approach to Assess Secondary Degeneration of Retinal Ganglion Cells

Ethanol-Induced Cervical Sympathetic Ganglion Block Applications for Promoting Canine Inferior Alveolar Nerve Regeneration Using an Artificial Nerve

Facial Nerve Surgery in the Rat Model to Study Axonal Inhibition and Regeneration

3D Kinematic Analysis for the Functional Evaluation in the Rat Model of Sciatic Nerve Crush Injury

Read Article