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Kapitel
- 00:01Titel
- 00:50Make Custom Stand
- 01:28Prepare Filter Papers for Culture
- 01:37Isolate Retina from Pig Eye
- 04:28Culturing of Isolated Retina
- 06:03Sectioning of Retina
- 07:31Immunohistochemistry
- 07:58Discussion
Here we describe a cost-effective technique for organotypic culture of adult porcine retina for seven days. Briefly, a sterile filter paper was used to lift the neural retina off from the RPE and place photoreceptor side up on an insert raised by a custom-made stand.
Tags
Organotypic CultureFull-thicknessAdult Porcine RetinaIn Vitro ModelsAnimal ExperimentsHuman Retinal InjuryDegenerative DiseaseCost-effective TechniqueAbattoirTrephine BladeNeural RetinaBruch’s MembraneRPE-choroid-sclera ExplantsSterile Filter PaperPhotoreceptor Side UpInsertCustom-made StandCulture MediumNative Retinal StructureMorphological StudiesPharmacological StudiesBiochemical Studies
