/
/
Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
This content is Free Access.
Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
17,117 Views
•
15:41 min
•
December 02, 2010
DOI:
Summary
Automatically generated
Adhesive micropatterns that normalize cellular architecture can be used to increase sensitivity in the detection of drug effects, improve reproducibility and simplify automated image acquisition and analysis. Such technology will benefit drug/siRNA screening assays, performed on conventional cell culture supports and consequently suffering from excessive cell-to-cell variability.
Explore More Videos
VisualizationQuantitative AnalysisDrug EffectsMicropatterned CellsHCA (High Content Analysis)Adherent Cell LinesHomogenous SubstrateCell ShapeMorphologyVerhaltenCell-to-cell VarianceMicropatternsShape NormalizationInternal PolarityCYTOOMicropatterning TechnologyCell SeedingDrug TreatmentFixation And StainingAutomated AcquisitionAutomated Image ProcessingData AnalysisCell CytoskeletonActin Meshwork
Read Article
Cite this Article
Degot, S., Auzan, M., Chapuis, V., Béghin, A., Chadeyras, A., Nelep, C., Calvo-Muñoz, M. L., Young, J., Chatelain, F., Fuchs, A. Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells. J. Vis. Exp. (46), e2514, doi:10.3791/2514 (2010).
Copy