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Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells
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December 02, 2010
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Adhesive micropatterns that normalize cellular architecture can be used to increase sensitivity in the detection of drug effects, improve reproducibility and simplify automated image acquisition and analysis. Such technology will benefit drug/siRNA screening assays, performed on conventional cell culture supports and consequently suffering from excessive cell-to-cell variability.
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VisualizationQuantitative AnalysisDrug EffectsMicropatterned CellsHCA (High Content Analysis)Adherent Cell LinesHomogenous SubstrateCell ShapeMorphology行为学Cell-to-cell VarianceMicropatternsShape NormalizationInternal PolarityCYTOOMicropatterning TechnologyCell SeedingDrug TreatmentFixation And StainingAutomated AcquisitionAutomated Image ProcessingData AnalysisCell CytoskeletonActin Meshwork
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Degot, S., Auzan, M., Chapuis, V., Béghin, A., Chadeyras, A., Nelep, C., Calvo-Muñoz, M. L., Young, J., Chatelain, F., Fuchs, A. Improved Visualization and Quantitative Analysis of Drug Effects Using Micropatterned Cells. J. Vis. Exp. (46), e2514, doi:10.3791/2514 (2010).
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