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在哺乳动物细胞通过显微注射mRNA的核出口动力学分析
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December 04, 2010
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在这里，我们描述了一个实验，采用显微注射的力量，再加上用荧光<em>原位</em>杂交为了准确地测量哺乳动物体细胞中的mRNA的核出口动力学。
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MRNA Nuclear Export KineticsMammalian CellsMicroinjectionEukaryotesCytoplasmTranslation MachineryXenopus OocytesYeastDrosophila Derived S2 Cell LineKinetic DataFluorescent In Situ Hybridization (FISH)Splicing Dependent MannerSignal Sequence Coding Region (SSCR)In Vitro Synthesized MRNAPlasmid DNASub-cellular Localization
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Gueroussov, S., Tarnawsky, S. P., Cui, X. A., Mahadevan, K., Palazzo, A. F. Analysis of mRNA Nuclear Export Kinetics in Mammalian Cells by Microinjection. J. Vis. Exp. (46), e2387, doi:10.3791/2387 (2010).
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