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Hücresel lizatları Multiprotein Kompleksleri Analizi Mavi Native Poliakrilamid Jel Elektroforez (BN-PAGE)
JoVE Journal
Biologie
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JoVE Journal Biologie
Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates
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Hücresel lizatları Multiprotein Kompleksleri Analizi Mavi Native Poliakrilamid Jel Elektroforez (BN-PAGE)

129,201 Views

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12:03 min

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February 24, 2011

DOI:

10.3791/2164-v

DOI:
10.3791/2164-v

•

12:03 min
February 24, 2011

•

66 Views
, ,
1Spemann Graduate School of Biology and Medicine (SGBM),University of Freiburg, 2Centre for Biological Signalling Studies (bioss) and Biology III, Faculty of Biology,University of Freiburg, 3Department of Molecular Immunology,Max-Planck-Institute of Immunology and Epigenetics

Summary

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Bu video, mavi yerli poliakrilamid jel elektroforezi (BN-PAGE) multiprotein kompleksler (MPCs) karakterizasyonu açıklar. Birinci boyut, diyaliz hücresel lizatları bireysel MPCs tanımlamak için BN-PAGE ile ayrılır. Ikinci bir boyut SDS-PAGE, faiz MPCs daha immün bileşenleri analiz alt gruba ayrılır.

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Blue Native Polyacrylamide Gel ElectrophoresisBN-PAGEMultiprotein ComplexesCell SignallingProtein-protein Interaction NetworksNative ConditionsResolutionGel FiltrationSucrose Density UltracentrifugationMPC SizeCompositionRelative AbundanceHydrodynamic Size And ShapePolyacrylamide MatrixCellular LysatesLysate DialysisFirst Dimension BN-PAGESecond Dimension SDS-PAGE

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Fiala, G. J., Schamel, W. W. A., Blumenthal, B. Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) for Analysis of Multiprotein Complexes from Cellular Lysates. J. Vis. Exp. (48), e2164, doi:10.3791/2164 (2011).

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