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SNP-Guided 5-FU Therapy in Gastric Cancer Using Ion Torrent Sequencing

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PCR Amplification and Adaptor Ligation of the Target Sequence for Multi-Gene SNP Detection in Gastric Cancer

JoVE Journal
Medizin

This content is Free Access.

JoVE Journal Medizin

PCR Amplification and Adaptor Ligation of the Target Sequence for Multi-Gene SNP Detection in Gastric Cancer

PCR Amplification and Adaptor Ligation of the Target Sequence for Multi-Gene SNP Detection in Gastric Cancer

SNP-Guided 5-FU Therapy in Gastric Cancer Using Ion Torrent Sequencing

DOI:

10.3791/201593-v

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01:37 min

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May 10, 2024

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Kaihua Huang, Xiao Yan, Zhicheng Huang, Huayuan Liang, Zhiwei Li, Minghao Wang, Yong Wan, Suihai Wang⁴, Liying Zhao

¹Nanfang Hospital, Southern Medical University, ²Research and Development Department,Guangzhou Darui Biotechnology Co., Ltd, ³Key Laboratory of Antibody Engineering of Guangdong Higher Education Institutes, School of Laboratory Medicine and Biotechnology,Southern Medical University, ⁴Provincial Key Laboratory of Immune Regulation and Immunotherapy,Southern Medical University

Tags

PCR Amplification Adaptor Ligation Multi-gene SNP Detection Gastric Cancer Custom Made Detection Kit Thermal Cycler Primer Digestion Enzyme Adapter Connecting Reagents Ligation Reagents

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