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SNP-Guided 5-FU Therapy in Gastric Cancer Using Ion Torrent Sequencing
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PCR Amplification and Adaptor Ligation of the Target Sequence for Multi-Gene SNP Detection in Gastric Cancer
JoVE 杂志
医学
This content is Free Access.
JoVE 杂志 医学
PCR Amplification and Adaptor Ligation of the Target Sequence for Multi-Gene SNP Detection in Gastric Cancer

PCR Amplification and Adaptor Ligation of the Target Sequence for Multi-Gene SNP Detection in Gastric Cancer

SNP-Guided 5-FU Therapy in Gastric Cancer Using Ion Torrent Sequencing

DOI:
10.3791/201593-v

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01:37 min

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May 10, 2024

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, , , , , , , ,
1Nanfang Hospital, Southern Medical University, 2Research and Development Department,Guangzhou Darui Biotechnology Co., Ltd, 3Key Laboratory of Antibody Engineering of Guangdong Higher Education Institutes, School of Laboratory Medicine and Biotechnology,Southern Medical University, 4Provincial Key Laboratory of Immune Regulation and Immunotherapy,Southern Medical University

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PCR AmplificationAdaptor LigationMulti-gene SNP DetectionGastric CancerCustom Made Detection KitThermal CyclerPrimer Digestion EnzymeAdapter Connecting ReagentsLigation Reagents

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