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Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
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September 03, 2009
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We present a method of targeted ancient DNA sequence retrieval, which we used to reconstruct the complete mitochondrial genomes of five Neandertal individuals. Comparison of these sequences with present day humans suggests that Neandertals had a long term low effective population size.
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Targeted DNA Sequence RetrievalHeavily Degraded DNA SourcesMicrobial DNA ContaminationAncient BonesSample Destruction ReductionSequencing Demands ReductionDirect PCRShotgun SequencingComplete Mitochondrial DNA (mtDNA) GenomesNeandertalsGeographic RangeMtDNA Genetic DiversityLate NeandertalsContemporary Modern HumansEffective Population Size
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Briggs, A. W., Good, J. M., Green, R. E., Krause, J., Maricic, T., Stenzel, U., Pääbo, S. Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources. J. Vis. Exp. (31), e1573, doi:10.3791/1573 (2009).
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