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Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources
JoVE 杂志
生物学
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JoVE 杂志 生物学
Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

Primer Extension Capture: Targeted Sequence Retrieval from Heavily Degraded DNA Sources

DOI:
10.3791/1573-v

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15:28 min

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September 03, 2009

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, , , , , ,
1Max-Planck Institute for Evolutionary Anthropology, Leipzig

Chapters

  • 00:00Title
  • 01:03Introduction
  • 01:39Amplification of Primary DNA LIbrary by PCR
  • 04:41Primer Extension
  • 08:13Extension Product Capture
  • 12:17Capture Product Amplification
  • 12:52Representative Results: PEC Retrieval of Neanderthal Mitochondrial Genomes
  • 14:45Conclusion

Summary

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We present a method of targeted ancient DNA sequence retrieval, which we used to reconstruct the complete mitochondrial genomes of five Neandertal individuals. Comparison of these sequences with present day humans suggests that Neandertals had a long term low effective population size.

Tags

Targeted DNA Sequence RetrievalHeavily Degraded DNA SourcesMicrobial DNA ContaminationAncient BonesSample Destruction ReductionSequencing Demands ReductionDirect PCRShotgun SequencingComplete Mitochondrial DNA (mtDNA) GenomesNeandertalsGeographic RangeMtDNA Genetic DiversityLate NeandertalsContemporary Modern HumansEffective Population Size

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