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Biologie
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由Stemgent人类TF慢病毒重新编程人类成纤维细胞生成诱导多能干细胞
JoVE Journal
Biologie
This content is Free Access.
JoVE Journal
Biologie
Generation of Induced Pluripotent Stem Cells by Reprogramming Human Fibroblasts with the Stemgent Human TF Lentivirus Set
Please note that all translations are automatically generated.
Click here for the English version.
由Stemgent人类TF慢病毒重新编程人类成纤维细胞生成诱导多能干细胞
DOI:
10.3791/1553-v
•
12:10 min
•
December 08, 2009
•
Dongmei Wu
,
Brad Hamilton
,
Charles Martin
,
Yan Gao
,
Mike Ye
,
Shuyuan Yao
1
Research and Development
,
Stemgent
Kapitel
00:00
Titel
00:09
Introduction
01:05
Preparing Mouse Embryonic Fibroblast (MEF) Conditioned Medium
02:39
Transducing Human-Foreskin-Fibroblast (BJ) Cells with Stem-Cell-Specific Transcription Factors
06:33
Selecting and Passaging Induced Pluripotent Stem Cell Colonies
07:33
Staining Resulting Colonies for Markers of Pluripotency
09:43
Representative Results: Induced Pluripotent Stem Cell Colonies Morphologically Resemble Stem-Cells and Express Markers of Pluripotency
11:07
Conclusion
Summary
Automatische Übersetzung
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Automatische Übersetzung
我们展示了为生成诱导多能干的干细胞,人类体细胞用慢病毒介导的人为因素OCT4,SOX2,NANOG和Lin28交付协议。多能性证实了形态学和胚胎干(ES)细胞特异性标志物的存在。
Tags
Induced Pluripotent Stem Cells
Reprogramming
Human Fibroblasts
Stemgent Human TF Lentivirus Set
Retrovirus-mediated Delivery
Oct4
Sox2
C-Myc
Klf4
Pluripotent State
Mouse Fibroblasts
Human Somatic Cells
IPS Cells
Transcription Factors
Retroviral Vectors
James Thomson
Nanog
Lin28
ES Cells
Morphology
Proliferation
Differentiation
Patient-specific IPS Cells
Personalized Regenerative Medicine Therapies
Article
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文化和维护人类胚胎干细胞
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