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蛍光顕微鏡による可視化単分子のDNAの複製
JoVE Journal
Biologie
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JoVE Journal Biologie
Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
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蛍光顕微鏡による可視化単分子のDNAの複製

22,148 Views

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15:57 min

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October 09, 2009

DOI:

10.3791/1529-v

DOI:
10.3791/1529-v

•

15:57 min
October 09, 2009

•

8 Views
, ,
1Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School

Summary

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このプロトコルは、リアルタイムで個々のreplisomesによってDNA複製を視覚化するためのシンプルな単一分子蛍光顕微鏡の技法を示しています。

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Cite this Article

Tanner, N. A., Loparo, J. J., van Oijen, A. M. Visualizing Single-molecule DNA Replication with Fluorescence Microscopy. J. Vis. Exp. (32), e1529, doi:10.3791/1529 (2009).

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