Lab coat |
|
|
protection |
SensiCareIce powder-free Nitrile Exam gloves |
Medline Industries, Inc, Mundelein, IL 60060, USA |
486802 |
sample protection |
Eppendorf Reference 2 pipette, 0.5-10µL |
Eppendorf France SAS, F-78360 Montesson, France |
4920000024 |
sample pipetting |
Eppendorf Reference 2 pipette, 20-100µL |
Eppendorf France SAS, F-78360 Montesson, France |
4920000059 |
sample pipetting |
Eppendorf Reference 2 pipette, 100-1000µL |
Eppendorf France SAS, F-78360 Montesson, France |
4920000083 |
sample pipetting |
TipOne 10µL Graduated, filter tip |
Starlab GmbH, D-22926 Ahrenburg, Germany |
S1121-3810 |
sample pipetting |
TipOne 1-100µL bevelled, filter tip |
Starlab GmbH, D-22926 Ahrenburg, Germany |
S1120-1840 |
sample pipetting |
ART 1000E Barrier Tip |
Thermo Fischer Scientific , F-67403 Illkirch, France |
2079E |
sample pipetting |
Eppendorf Safe-Lock Tubes, 1.5 mL, Eppendorf Quality |
Eppendorf France SAS, F-78360 Montesson, France |
30120086 |
mix |
Vortex-Genie 2 |
Scientific Industries, Inc, Bohemia, NY 111716, USA |
SI-0236 |
mix |
Mikro 200 centrifuge |
Hettich Zentrifugen, D-78532, Germany |
0002020-02-00 |
centrifugation |
Multipette E3 |
Eppendorf France SAS, F-78360 Montesson, France |
4987000010 |
distribution |
Light Cycler 480 multiwell plate 96, white |
Roche Diagnostics GmbH, D-68305 Mannheim, Germany |
4729692001 |
reaction place |
Light Cycler 480 sealing foil |
Roche Diagnostics GmbH, D-68305 Mannheim, Germany |
4429757001 |
coverage |
LightCycler 480 Instrument II, 96-well |
Roche Diagnostics GmbH, D-68305 Mannheim, Germany |
05015278001 |
high resolution melting polymerase chain reaction |
Heraeus Megafuge 11R centrifuge |
Thermo Fischer Scientific , F-67403 Illkirch, France |
75004412 |
centrifugation |
LightCycler 480 High Resolution Melting Master |
Roche Diagnostics GmbH, D-68305 Mannheim, Germany |
04909631001 |
reaction reagents |
CN3 primer: 5'ggccttagacttctcctgc 3' |
Eurogentec Biologics Division, B4102 Seraing, Belgium |
|
reaction reagent |
CN3re primer: 5'gttgacaaattggcggcttcg 3' |
Eurogentec Biologics Division, B4102 Seraing, Belgium |
|
reaction reagents |
light cycler 480 SW 1.5.1 software |
Roche Diagnostics GmbH, D-68305 Mannheim, Germany |
|
software used for HRM-PCR CR1 polymorphism data analysis |