zPDX-Analysis of Invasiveness: Investigating Invasive Behavior of Metastatic Cancer Cells in Zebrafish Embryo Xenografts

Published: April 30, 2023

Abstract

Source: Ren, J. et al. Invasive Behavior of Human Breast Cancer Cells in Embryonic Zebrafish. J. Vis. Exp. (2017).

This video describes a microinjection method for breast cancer cell delivery into the perivitelline space of transgenic zebrafish embryos. After incubation, we visualize the embryo under a fluorescent microscope to observe cancer cell invasion, dissemination, and metastasis.

Protocol

1. Image and Analyze the Metastatic Process Collect several anesthetized embryos with a wide-tip Pasteur pipette and transfer them to the glass bottom of a polystyrene dish. Remove excess water and keep a limited amount of egg water. Manipulate the embryo into position with a hair loop tool and place a cover on top of the glass. Use an inverted confocal microscope in combination with water-immersion or long-distance dry objectives. Position the embryo such that the region …

Offenlegungen

The authors have nothing to disclose.

Materials

Wide-tip Pasteur pipette (0.5-20 µL) Eppendorf F276456I
Confocal microscope Leica SP5 STED
Stereo microscope Leica MZ16FA
Tg (fli:EGFP) zebrafish strain Kindly provided by Dr. Ewa SnaarJagalska (Institute of Biology, Leiden University, Leiden, The Netherlands)
Polystyrene dish with glass bottom WillCo GWST-5040
Fluorescent stereo microscope Leica M165 FC
Tricaine (3-aminobenzoic acid) SigmaAldrich A-5040

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Diesen Artikel zitieren
zPDX-Analysis of Invasiveness: Investigating Invasive Behavior of Metastatic Cancer Cells in Zebrafish Embryo Xenografts. J. Vis. Exp. (Pending Publication), e20219, doi: (2023).

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