JOVE JOURNAL

Biochemie

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Nonradioactive Assay to Measure Polynucleotide Phosphorylation of Small Nucleotide Substrates

¹Signal Transduction Laboratory, National Institute of Environmental Health Sciences, Department of Health and Human Services,National Institutes of Health

Journal (Biochemistry)

A Hydrogen-Deuterium Exchange Mass Spectrometry (HDX-MS) Platform for Investigating Peptide Biosynthetic Enzymes

Yeganeh Habibi¹, Christopher J. Thibodeaux¹

¹Department of Chemistry,McGill University

Journal (Biochemistry)

Paper-Based Preconcentration and Isolation of Microvesicles and Exosomes

Dohwan Lee*¹, Kyung Wook Wee*¹, Hyerin Kim*¹, Sung Il Han¹, Seungmin Kwak^1,2, Dae Sung Yoon³, Jeong Hoon Lee¹

¹Department of Electrical Engineering,Kwangwoon University, ²Korea Institute of Science and Technology (KIST), ³School of Biomedical Engineering,Korea University

Journal (Biochemistry)

A Plasma Sample Preparation for Mass Spectrometry using an Automated Workstation

Qin Fu¹, Casey W. Johnson¹, Bhagya K. Wijayawardena², Michael P. Kowalski², Miranda Kheradmand², Jennifer E. Van Eyk¹

¹Advanced Clinical Biosystems Institute, Smidt Heart institute,Cedars Sinai Medical Center, ²Beckman Coulter Life Sciences

Journal (Biochemistry)

Detection of Protein S-Acylation using Acyl-Resin Assisted Capture

Ritika Tewari*¹, Savannah J. West*^1,2, Bieerkehazi Shayahati¹, Askar M. Akimzhanov¹

¹Department of Biochemistry and Molecular Biology,McGovern Medical School at UT Health, ²MD Anderson UT Health Graduate School

Journal (Biochemistry)

In Vivo Hydroxyl Radical Protein Footprinting for the Study of Protein Interactions in Caenorhabditis elegans

Jessica A. Espino¹, Lisa M. Jones¹

¹Department of Pharmaceutical Sciences,University of Maryland

Journal (Biochemistry)

Acyl-PEGyl Exchange Gel Shift Assay for Quantitative Determination of Palmitoylation of Brain Membrane Proteins

David J. Speca¹, Elva Diaz¹

¹Department of Pharmacology,UC Davis School of Medicine

Journal (Biochemistry)

Characterization of Amyloid Structures in Aging C. Elegans Using Fluorescence Lifetime Imaging

Maria Lucia Pigazzini*^1,2, Christian Gallrein*¹, Manuel Iburg*¹, Gabriele Kaminski Schierle³, Janine Kirstein^1,4

¹Leibniz Research Institute for Molecular Pharmacology im Forschungsverbund Berlin, ²NeuroCure Cluster of Excellence,Charité - Universitätsmedizin Berlin, ³Molecular Neuroscience Group, Department of Chemical Engineering and Biotechnology,University of Cambridge, ⁴Cell Biology,University of Bremen

Journal (Biochemistry)

Enrichment of Mammalian Tissues and Xenopus Oocytes with Cholesterol

Alexandria Slayden¹, Kelsey North¹, Shivantika Bisen¹, Alex M. Dopico¹, Anna N. Bukiya¹, Avia Rosenhouse-Dantsker²

¹Department of Pharmacology, Addiction Science and Toxicology,The University of Tennessee HSC, ²Department of Chemistry,University of Illinois at Chicago

Journal (Biochemistry)

Detection of Protein Ubiquitination Sites by Peptide Enrichment and Mass Spectrometry

Karel Bezstarosti¹, Lennart van der Wal¹, Jeroen A. A. Demmers¹

¹Proteomics Center,Erasmus University Medical Center

Journal (Biochemistry)

Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson’s Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation

Ying Fan*¹, Francesca Tonelli*¹, Shalini Padmanabhan², Marco A.S. Baptista², Lindsey Riley², Danielle Smith³, Connie Marras⁴, Andrew Howden⁵, Dario R. Alessi¹, Esther Sammler^1,6

¹MRC Protein Phosphorylation and Ubiquitylation Unit, School of Life Sciences,University of Dundee, ²The Michael J. Fox Foundation for Parkinson's Research, ³Department of Medical and Molecular Genetics,Indiana University School of Medicine, ⁴Morton and Gloria Shulman Movement Disorders Centre and the Edmond J. Safra Program in Parkinson's Disease, Toronto Western Hospital,University of Toronto, ⁵Division of Cell Signalling and Immunology, School of Life Sciences,University of Dundee, ⁶Division of Molecular and Clinical Medicine, School of Medicine, Ninewells Hospital and Medical School,University of Dundee

Journal (Biochemistry)

Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

Filip Pamula^1,2, Jonas Mühle¹, Alain Blanc³, Rony Nehmé⁴, Patricia C. Edwards⁴, Christopher G. Tate⁴, Ching-Ju Tsai¹

¹Laboratory of Biomolecular Research,Paul Scherrer Institute, ²Department of Biology,ETH Zürich, ³Center for Radiopharmaceutical Sciences,Paul Scherrer Institute, ⁴Laboratory of Molecular Biology,Medical Research Council

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